pET-32a（+）
pET-32a(+)D0064 pET-32a(+) NobleRyder 25ug(0.5OD) 560.00载体类型：大肠杆菌表达载体 载体大小： 5900 bp 载体宿主：大肠杆菌（E.coli） 细菌抗性：氨苄青霉素（Amp） 5' 测序引物：S Tag primer3' 测序引物：T7 terminator primer
pET-32a（+）
D0064 pET-32a（+） NobleRyder 25ug（0.5OD） 560.00
载体类型：大肠杆菌表达载体
载体大小： 5900 bp
载体宿主：大肠杆菌（E.coli）
细菌抗性：氨苄青霉素（Amp）
5' 测序引物：S Tag primer
3' 测序引物：T7 terminator primer
载体简介：
The pET-32 series is designed for cloning and high-level expression of peptide sequences fusedwith the 109aa Trx Tag thioredoxin protein . Cloning sites are available for producing fusionproteins also containing cleavable His Tag and S Tag sequences for detection and puri cation.Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression regionof the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented
so that infection with helper phage will produce virions containing single-stranded DNA that corre-sponds to the coding strand. Therefore, single-stranded sequencing should be performed using theT7 terminator primer.
D0084 | BL21（DE3）pLysS受体菌 | NobleRyder | 1ml | 100.00 |
D0083 | BL21（DE3）受体菌 | NobleRyder | 1ml | 100.00 |
D0080 | DH5α受体菌 | NobleRyder | 1ml | 100.00 |
D0085 | HB101受体菌 | NobleRyder | 1ml | 100.00 |
D0098 | IPTG 溶液 （200mg/ml） | NobleRyder | 1ml/5ml | 60.00/160.00 |
D0082 | JM109受体菌 | NobleRyder | 1ml | 100.00 |
D0110 | LB培养基 | NobleRyder | 10L | 260.00 |
D0063 | PBR322 | NobleRyder | 25ug（0.5OD） | 100.00 |
D0064 | NobleRyder | 25ug（0.5OD） | 560.00 | |
D0061 | pUC18 | NobleRyder | 25ug | 100.00 |
D0062 | PUC19 | NobleRyder | 25ug（0.5OD） | 100.00 |
D0081 | *0受体菌 | NobleRyder | 1ml | 100.00 |
D0096 | X-gal溶液（20mg/ml） | NobleRyder | 1ml | 40.00 |
D0099 | 氨苄青霉素溶液（100mg/ml） | NobleRyder | 10ml | 100.00 |
