北京诺博莱德科技有限公司

Zeocin博莱霉素 优势产品

 博莱霉素 Zeocin

产品简介

Zeocin™ 试剂是博来霉素抗生素家族的一个成员。其抗性由编码一种 13,665 道尔顿蛋白质的 Sh ble 基因赋予。在表达这种蛋白质的细胞中，Zeocin™ 无法结合和切割细胞 DNA。 Zeocin™ 对大多数好氧细胞有效，可用于选择哺乳动物和昆虫细胞系、酵母以及细菌。 选择细胞时，Zeocin™ 的用量为50-2000 ug/ml（通常 300 ug/ml），具体取决于细胞类型。 

C₅₅H₈₃N₁₉O₂₁S₂Cu 

式量： 1137.41 克/摩尔

Zeocin™ 是 CAYLA 的商标。

ble 抗性基因受一个或多个美国（号 5,021,344 和 5,118,620）以及相关外国保护，Cayla 拥有权，Invitrogen 已从 Cayla 获得使用许可。 该产品的销售于研究目的。
　　 
使用:
　　●液体Zeocin可用于细胞培养。 
　　●用于筛选转化株的Zeocin浓度根据pH值和盐浓度改变。 
　　●pH越高，盐浓度越低Zeocin活性越大。 
　　●普通大肠杆菌菌株筛选可在25＆micro；g／ml zeocin浓度的低盐LB琼脂培养基中进行，pH调至7.5。 
　　●动物细胞筛选：根据不同细胞系从100到750＆micro；g／ml。 
　　●植物细胞筛选：根据不同的细胞种类和培养基从50到250＆micro；g／ml。 
　　●酵母筛选：取决于种属和培养基，S。cerevisiaeSh ble转化株在50＆micro；g／ml浓度的YPD培养基中筛选，pH7.0.304015.

Invitrogen相关资料如下:

Zeocin™ Selection Reagent

Cat. No. R250-01

Description

Zeocin™ Reagent is a member of the bleomycin antibiotic family. Resistanceis conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cellsexpressing the protein, Zeocin™ is prevented frombinding and cleaving the cellular DNA. Zeocin™ iseffective on most aerobic cells and can be used for selection in mammalian andinsect cell lines, yeast, and bacteria. The amount of Zeocin™required for selection will vary between 50-2000 ug/ml (typically300 ug/ml) depending on the cell type.

C55H83N19O21S2Cu

Formula Weight: 1137.41 g/mole

Zeocin™ is a trademark of CAYLA.

The ble resistance gene is the subject ofone or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreignequivalents owned by Cayla and licensed to Invitrogen. This product is sold forresearch purposes only. For commercial license information, please contact:Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA92008.

General Specifications

Concentration:       100 mg⁄mL

Brand:       Zeocin™

Form:       Liquid

Product Size:       1 g

ValidatedApplication:       Eukaryotic Selection⁄Stable Cell Line Generation

Bacterial Selection

Contents & Storage

Zeocin™ is provided in solution at a concentration of 100 mg/ml. The 1 gramsize is supplied in 8 vials. The 5 gram size is supplied in one vial. Store Zeocin™ at -20°C inthe dark. Zeocin™ is guaranteed stable for 6 months when properly stored.